The effects of a full-scale anaerobic side-stream reactor on sludge decay and biomass activity.

A full-scale anaerobic side-stream reactor (ASSR) for sludge reduction was monitored in terms of sludge production and compared with the previous conventional activated sludge configuration (CAS). A detailed solid mass balance was calculated on the whole full-scale plant to estimate the sludge reduction associated with the ASSR. The activity of the biomass, which undergoes alternation of aerobic and anaerobic conditions, was investigated by the respirometric test. The ASSR promoted a reduction of heterotrophic biomass activity and the substrate consumption rate in the activated sludge implemented with ASSR (AS + ASSR) was 36% smaller than in the CAS period. The solid mass balance indicated a sludge reduction of 28%. During the 270-day operation, the observed sludge yield passed from 0.438 kgTSS/kgCOD in the CAS to 0.315 in the AS + ASSR configuration. The solubilization of chemical oxygen demand (COD), NH4 +-N and orthophosphate were verified under anaerobic conditions. The results suggest that the possible mechanisms of sludge reduction were the increase of the system sludge retention time (SRT) by ASSR addition, and the reduction in heterotrophic biomass activity added to the organic compounds' hydrolysis.

Effect of COD/N ratio on N2O production during nitrogen removal by aerobic granular sludge.

N2O-production was investigated during nitrogen removal using aerobic granular sludge (AGS) technology. A pilot sequencing batch reactor (SBR) with AGS achieved an effluent in accordance with national discharge limits, although presented a nitrite accumulation rate of 95.79% with no simultaneous nitrification-denitrification. N2O production was 2.06 mg L-1 during the anoxic phase, with N2O emission during air pulses and the aeration phase of 1.6% of the nitrogen loading rate. Batch tests with AGS from the pilot reactor verified that at the greatest COD/N ratio (1.55), the N2O production (1.08 mgN2O-N L-1) and consumption (up to 0.05 mgN2O-N L-1), resulted in the lowest remaining dissolved N2O (0.03 mgN2O-N L-1), stripping the minimum N2O gas (0.018 mgN2O-N L-1). Conversely, the carbon supply shortage, under low C/N ratios, increased N2O emission (0.040 mgN2O-N L-1), due to incomplete denitrification. High abundance of ammonia-oxidizing and low abundance of nitrite-oxidizing bacteria were found, corroborating the fact of partial nitrification. A denitrifying heterotrophic community, represented mainly by Pseudoxanthomonas, was predominant in the AGS. Overall, the AGS showed stable partial nitrification ability representing capital and operating cost savings. The SBR operation flexibility could be advantageous for controlling N2O emissions, and extending the anoxic phase would benefit complete denitrification in cases of low C/N influents.

Ecology of duckweed ponds used for nutrient recovery from wastewater.

The microorganism community that grows under duckweed shelter can play an important role on treatment processes. Therefore, the present study aimed to assess the zooplankton dynamic and microbial community in duckweed ponds (DPs) applied for domestic wastewater treatment under open field conditions. A pilot system comprised of two DPs in series (DP1 and DP2), with 10 m2 each, received domestic wastewater through a flow rate of 200 L·day-1. Thus, the system was monitored during 314 days through samples collected and analysed weekly. Also, the zooplankton organisms were identified and quantified. DNA sequencing was performed in order to identify the bacterial populations. The findings showed a high efficiency of nutrient removal with 93% and 91% of total phosphorus and total nitrogen, respectively. A high density of microcrustaceans was observed in DP1 reaching 4,700 org.100 mL-1 and rotifers (over than 32,000 org.100 mL-1) in DP2, that could be related to the low suspended solids concentration (<30 mg·L-1) and turbidity (<10 NTU). The bacterial community showed a strong heterogeneity between samples collected along the seasons. Through these findings, it is possible to realise that the understanding of ecology could help to enhance the operation and designs of DPs.

Anaerobic side-stream reactor for excess sludge reduction: 5-year management of a full-scale plant.

The long-term performances of a full-scale anaerobic side-stream reactor (ASSR) aimed at sludge reduction have been monitored for the first time, in comparison with a conventional activated sludge process (CAS). The plant was integrated with an ASSR treatment of 2293-3293 m(3). Operational parameters in the ASSR were: ORP -250 mV, interchange ratio of 7-10%, hydraulic retention time of 7 d. No worsening of effluent quality was observed in the ASSR configuration and removal efficiency of COD and NH4 was above 95%. A slight increase in the Sludge Volume Index did not cause worsening in effluent solids concentration. The observed sludge yield (Yobs) passed from 0.44 kgTSS/kgCOD in the CAS to 0.35 in the ASSR configuration. The reduction of Yobs by 20% is lower than expected from the literature where sythetic wastewater is used, indicating that sludge reduction efficiency is largely affected by inert mass fed with influent real wastewater. An increase by 45% of the ASSR volume did not promote a further reduction of Yobs, because sludge reduction is affected not solely by endogenous decay but also by other factors such as interchange ratio and aerobiosis/anaerobiosis alternation.

Valuation of OSA process and folic acid addition as excess sludge minimization alternatives applied in the activated sludge process.

The aim of this study was to investigate the ability of the oxic-settling-anaerobic (OSA)-process and the folic acid addition applied in the activated sludge process to reduce the excess sludge production. The study was monitored during two distinct periods: activated sludge system with OSA-process, and activated sludge system with folic acid addition. The observed sludge yields (Yobs) were 0.30 and 0.08 kgTSS kg(-1) chemical oxygen demand (COD), control phase and OSA-process (period 1); 0.33 and 0.18 kgTSS kg(-1) COD, control phase and folic acid addition (period 2). The Yobs decreased by 73 and 45% in phases with the OSA-process and folic acid addition, respectively, compared with the control phases. The sludge minimization alternatives result in a decrease in excess sludge production, without negatively affecting the performance of the effluent treatment.

Aerobic granular sludge technology and nitrogen removal for domestic wastewater treatment.

This study evaluated aerobic granulation and nitrogen removal via assimilation, nitrification, and denitrification of a system fed with real domestic wastewater. The granulation process was complete after 160 days of operation. The mature granules had an almost spherical structure, an average size of 473.0 µm, and a good settling ability (SVI30 of 75.6 mL g⁻¹). Ammonium assimilation for cell growth varied between 3.5 and 64.6% during reactor start-up. After granule formation, assimilation accounted for less than 5% and nitrogen was mainly removed by partial nitrification up to nitrite, followed by denitrification via nitrite. Average efficiencies of 86.6% for nitrification, 59.5% for denitrification, and 60.5% for total nitrogen were obtained in this period. The assimilation ability of the mature granules grown on domestic wastewater was lower than the commonly reported results obtained for synthetic granules.

Concerning the role of cell lysis-cryptic growth in anaerobic side-stream reactors: the single-cell analysis of viable, dead and lysed bacteria.

In the Anaerobic Side-Stream Reactor (ASSR), part of the return sludge undergoes alternating aerobic and anaerobic conditions with the aim of reducing sludge production. In this paper, viability, enzymatic activity, death and lysis of bacterial cells exposed to aerobic and anaerobic conditions for 16 d were investigated at single-cell level by flow cytometry, with the objective of contributing to the understanding of the mechanisms of sludge reduction in the ASSR systems. Results indicated that total and viable bacteria did not decrease during the anaerobic phase, indicating that anaerobiosis at ambient temperature does not produce a significant cell lysis. Bacteria decay and lysis occurred principally under aerobic conditions. The aerobic decay rate of total bacteria (bTB) was considered as the rate of generation of lysed bacteria. Values of bTB of 0.07-0.11 d(-1) were measured in anaerobic + aerobic sequence. The enzymatic activity was not particularly affected by the transition from anaerobiosis to aerobiosis. Large solubilisation of COD and NH4(+) was observed only under anaerobic conditions, as a consequence of hydrolysis of organic matter, but not due to cell lysis. The observations supported the proposal of two independent mechanisms contributing equally to sludge reduction: (1) under anaerobic conditions: sludge hydrolysis of non-bacterial material, (2) under aerobic conditions: bacterial cell lysis and oxidation of released biodegradable compounds.

Consortia of microalgae and bacteria in the performance of a stabilization pond system treating landfill leachate.

This study treated sanitary landfill leachate and was conducted in a pilot-scale system composed of three serial ponds (P1, P2 and P3), followed by a rock filter, in order to evaluate the microbial consortium influence on system performance and to investigate microorganism dynamics in the process. The system was broken into three stages, with a continuous flow rate (Q = 200 L d⁻¹) for 43 weeks. The stages were as follows: conventional operation (stage I), 12 h aeration in P2 (stage II), and 18 h aeration in P2 (stage III). The results showed the possibilities for treating landfill leachate, presenting an average efficiency of 75% for both filtered biochemical oxygen demand and ammonium. At the end of stage III, the ammonium concentration was 6 mg L⁻¹, which is lower than that established by Brazilian regulations for wastewater discharge (CONAMA 430/2011). The aeration applied in P2 led to a change in the microbial consortia during the second and third stage, which influenced the quality of the final effluent. The best performance was seen in stage III, where the system showed high microbial diversity, including the presence of nitrifying bacteria.

Using full-scale duckweed ponds as the finish stage for swine waste treatment with a focus on organic matter degradation.

The rapid increase in the number of swine has caused pronounced environmental impacts worldwide, especially on water resources. As an aggregate, smallholdings have an important role in South American pork production, contributing to the net diffusion of pollution. Thus, duckweed ponds have been successfully used for swine waste polishing, mainly for nutrient removal. Few studies have been carried out to assess organic matter degradation in duckweed ponds. Hence, the present study evaluated the efficiency of two full-scale duckweed ponds for organic matter reduction of swine waste on small pig farms. Duckweed ponds, in series, received the effluent after an anaerobic biodigester and storage pond, with a flow rate of 1 m(3) day(-1). After 1 year of monitoring, an improvement in effluent quality was observed, with a reduction in biochemical oxygen demand (BOD) and total chemical oxygen demand (tCOD), respectively, of 94.8 and 96.7%, operating at a loading rate of approximately 27 kgBOD ha(-1) day(-1) and 131 kgCOD ha(-1) day(-1). Algae inhibition due to duckweed coverage was strongly observed in the pond effluent, where chlorophyll a and turbidity remained below 25 µg L(-1) and 10 NTU. Using the study conditions described herein, duckweed ponds were shown to be a suitable technology for swine waste treatment, contributing to the environmental sustainability of rural areas.

Nutrient recovery from swine waste and protein biomass production using duckweed ponds (Landoltia punctata): southern Brazil.

Brazil is one of the most important countries in pork production worldwide, ranking third. This activity has an important role in the national economic scenario. However, the fast growth of this activity has caused major environmental impacts, especially in developing countries. The large amount of nitrogen and phosphorus compounds found in pig manure has caused ecological imbalances, with eutrophication of major river basins in the producing regions. Moreover, much of the pig production in developing countries occurs on small farms, and therefore causes diffuse pollution. Therefore, duckweed pond have been successfully used in the swine waste polishing, generating further a biomass with high protein content. The present study evaluated the efficiency of two full scale duckweed ponds for the polishing of a small pig farm effluent, biomass yield and crude protein (CP) content. Duckweed pond series received the effluent from a biodigester-storage pond, with a flow rate of 1 m(3)/day (chemical oxygen demand rate = 186 kg/ha day) produced by 300 animals. After 1 year a great improvement of effluent quality was observed, with removal of 96% of total Kjeldahl nitrogen (TKN) and 89% of total phosphorus (TP), on average. Nitrogen removal rate is one of the highest ever found (4.4 g TKN/m(2) day). Also, the dissolved oxygen rose from 0.0 to 3.0 mg/L. The two ponds produced together over 13 tons of fresh biomass (90.5% moisture), with 35% of CP content, which represents a productivity of 24 tonsCP/ha year. Due to the high rate of nutrient removal, and also the high protein biomass production, duckweed ponds revealed, under the presented conditions, a great potential for the polishing and valorization of swine waste. Nevertheless, this technology should be better exploited to improve the sustainability of small pig farms in order to minimize the impacts of this activity on the environment.

Modelling waste stabilisation ponds with an extended version of ASM3.

In this paper an extended version of IWA's Activated Sludge Model No 3 (ASM3) was developed to simulate processes in waste stabilisation ponds (WSP). The model modifications included the integration of algae biomass and gas transfer processes for oxygen, carbon dioxide and ammonia depending on wind velocity and a simple ionic equilibrium. The model was applied to a pilot-scale WSP system operated in the city of Florianópolis (Brazil). The system was used to treat leachate from a municipal waste landfill. Mean influent concentrations to the facultative pond of 1,456 g(COD)/m(3) and 505 g(NH4-N)/m(3) were measured. Experimental results indicated an ammonia nitrogen removal of 89.5% with negligible rates of nitrification but intensive ammonia stripping to the atmosphere. Measured data was used in the simulations to consider the impact of wind velocity on oxygen input of 11.1 to 14.4 g(O2)/(m(2) d) and sun radiation on photosynthesis. Good results for pH and ammonia removal were achieved with mean stripping rates of 18.2 and 4.5 g(N)/(m(2) d) for the facultative and maturation pond respectively. Based on measured chlorophyll a concentrations and depending on light intensity and TSS concentration it was possible to model algae concentrations.

Utilization of a hybrid sequencing batch reactor (HSBR) as a decentralized system of domestic wastewater treatment.

This paper presents the experiments carried out in a hybrid sequencing batch reactor (HSBR), used for biological treatment of sewage. The HSBR was built in a cylindrical shape and made of stainless steel, with a volume of 1.42 m3. Besides the biomass in suspension, the reactor also carried fixed biomass (hybrid process), adhered in the support material. This consisted of a nylon net disposed in a grille for biofilm biomass adhesion. The reactor worked fully automated in operational cycles of maximum 8 hours each, presenting the following phases: filling, anoxic, aerobic, settle and draw of treated effluent, with 3 fillings per cycle. Increasing organic loads (0.14 to 0.51 kg TCOD/m3 day) and ammonium loads (0.002 to 0.006 kg NH4-N/m3.day) were tested. We monitored the reactor's performance by measuring the liquid phase (COD, pH, temperature, DO, nitrogen and phosphorus) during the cycles and by measuring the sludge through respirometric tests. The results obtained demonstrated TCOD removal efficiency between 73 and 96%, and ammonium removal efficiency between 50 and 99%. At the end of the cycles, the effluent presented ammonium concentration <20 mg/L, meeting the Brazilian environmental legislation standards (CONAMA 357/2005) regarding discharges into the water bodies. Respirometric tests showed biomass dependency on FCOD concentrations. Results have demonstrated the potential of this type of reactor for decentralized treatment of domestic wastewater.

Reduction of odors from a facultative pond using two different operating practices.

This paper presents the results of a proposed intervention to deal with the odor problems of a sewage treatment works (STW), which is located near a populated area. The STW consists of a facultative pond. Since this pond functions under close to anaerobic conditions, unpleasant odors are emitted. In this respect, two possible ways to deodorize the pond were evaluated. Firstly, the recirculation of effluent using 1/6 of the flow stream followed by aeration of the pond with a reduced power aerator. In order to study the efficiencies of the deodorization methodologies chemical analyses of the gases NH3 and H2S, olfactometric analyses and evaluation of the environmental perception of the population in relation to the odors originating from the STW, were carried out for each experimental situation. The results showed a significant reduction in odors when aeration with reduced power equipment was utilized in combination with recirculation of effluent in the pond. Reductions in emissions of H2S from 0.1345 mg/m3 to 0.0083 mg/m3 and of NH3 from 0.021 mg/m3 to 0.0073 mg/m3 were obtained. To analyze the behavior of the pond, its planktonic community was investigated, with a difference in species for the situations with and without odor being observed.

Evaluation of sludge from pond system for treatment of piggery wastes.

Stabilization ponds used for the treatment of piggery wastes accumulate sludge over time, which is commonly used in agriculture. The objective of this study was to evaluate the agronomic potential of this kind of sludge. The samplings were collected in two different phases. The first in two anaerobic ponds (AP1 and AP2) and in one facultative pond with 5 transverse baffles and, the second in the same facultative pond with aeration. The removed sludge of AP1 and AP2 was characterized as rich sludge in volatile solids and with low stabilization, there was a great accumulation of the total phosphorus in the sludge of AP2. The facultative pond presented greater retention of nutrients in the sludge in relation to the anaerobic ponds. The annual accumulation of sludge was 13.3 cm/year in the AP1 and 6.70 cm/year in the AP2, while in the pond with aeration this was on the average of 0.5 cm/year, due to the aeration regime. The sludge can be used as a fertilizer in agriculture, if the chemical characteristics of the soil are taken into account so as to avoid the accumulation of nutrients and damage to plants.

Biogas production, sludge accumulation and mass balance of carbon in anaerobic ponds.

This work concerned the application of anaerobic ponds for the primary treatment of urban wastewater in a Mediterranean climate. It was carried out on anaerobic ponds at large scale in Mèze (France). The anaerobic ponds constitute a good primary treatment with the removal of 55% of SS and 30% of BOD5, with a small surface area. The accumulation rate of sludge was only 0.017 m3/capita.year, due to their intensive anaerobic degradation. The anaerobic digestion reached equilibrium after one year of operation. The accumulation of sludge then showed seasonal variations with a substantial accumulation in winter and the digestion of the stock in summer. This change can be related to the influence of the temperature on methanogenesis. The production of biogas (83% CH4) was measured by gas collectors especially developed for this study and was also strongly dependent on temperature. The mass balance of carbon showed that 74% of the removed organic carbon was converted into CH4, 13% into dissolved inorganic carbon and 15% was stored in sludge. However, the anaerobic ponds presented a risk of creating odor nuisances with the emission of H2S.

Optimization of the treatment of piggery wastes in water hyacinth ponds.

This work investigates the optimal management of water hyacinth ponds for the improvement of piggery waste treatment. The optimal harvesting strategy for the water hyacinth was studied using a single mathematical model. The water hyacinth optimal harvesting problem was formulated as an optimal control problem that was solved by application of Pontryagin's Maximum Principle. The optimization of the water hyacinth control in the pond indicates that the plant density should be reduced whenever it reaches half of the maximum capacity for growth. Two experimental systems were used to validate the mathematical model, one in real scale and the other in pilot scale. The results demonstrated the feasibility of the proposed harvesting strategy. For example, a comparison of the total nitrogen removal in the different pilot ponds confirmed the modeling results, in that the performance of the pond maintained with 50% water hyacinth cover was better than the others.

Micronucleus induction in mussels exposed to okadaic acid.

Some toxins present in the marine environment are capable of inducing mutagenicity and/or carcinogenicity. Among these toxins, okadaic acid (OA) is gaining considerable interest since it induces DNA based modifications at low concentrations and accumulates in filter-feeding marine animals, including those used for human consumption. This study aims to evaluate the genotoxicity of OA in the haemocytes of the mussel Perna perna, using the micronucleus assay. Fifty-four mussels were separated into three groups of 18 animals. One group received 0.3 microg of OA diluted in 10 microl of ethanol and ultrapure water while the other groups were considered as controls and were exposed to a solvent plus seawater mixture. A significantly higher frequency of micronuclei was observed in haemocytes from the OA-exposed group. There were no statistical differences between the two control groups.

Influence of load distribution and recycle rate in step-fed facultative ponds.

This study presents the results of research on a wastewater treatment system with four identical facultative ponds in series with step-feeding and recirculation (SFFPR). Four modes of distribution of the influent were studied, which were (in percentages of the inflow per pond): C1 = 25/25/25/25; C2 = 50/20/20/10; C3 = 50/50/0/0; C4 = 100/0/0/0. The organic loading applied to the four ponds overall was around 200 kg BOD5/ha.d. The distribution C4 = 100/0/0/0 was selected for studying the recycle rate. Three recycling rates were studied: 0.5Q, 1Q and 2Q. COD and BOD5 removal efficiencies were high whatever the step-feed distribution. For the distribution C1 and C2, the four ponds were homogeneous. The equal distribution of organic loading rate in C2 distribution was most beneficial for algal growth. For piston pattern distributions (C3 and C4), the ponds receiving the highest loading showed a bacterial biomass higher than that of the primary production. Increasing the recirculation rate seemed to lead towards homogenisation of the ponds, while a decrease appeared to lead to their individualisation and increased their productivity. The SFFPR constitutes a stage of the treatment process which can replace the facultative stage; it produces a high effluent quality and decreases land area requirements.

Performance of a baffled facultative pond treating piggery wastes.

This paper shows the performance of a baffled facultative pond for the treatment of piggery wastes. The full-scale system is composed of an equalizer, one decanter (DP), two anaerobic ponds (LA1 and LA2), one facultative pond (LF), with five baffles, and one maturation pond with water hyacinths (LAG). The studies were conducted over a 12 month period in the west region of Santa Catarina, Brazil. The system was supplied daily with a volume of 3 m3/day of farm wastes. A good performance of the treatment system was obtained with average removal efficiencies of 98% for chemical oxygen demand, 93% for total solids, 98% for total phosphorus, 92% for total nitrogen, 7 log units of faecal coliforms and 5 log units of total coliforms. The facultative pond performed well, removing 43% of the chemical oxygen demand, 47% of total nitrogen and 54% of total phosphorus. It was found that the first baffle in the facultative pond was mainly responsible for the efficiency of this pond, and compared with another study the introduction of the baffles improved the removal efficiency by 20% for total phosphorus.